micropropagation of four cultivars (dargazi, natanzi, shahmiveh and williams) of pear (pyrus communis l.)

نویسندگان

سید ضیاء نصرتی

ذبیح اله زمانی

مصباح بابالار

چکیده

three separate experiments were conducted to investigate micropropagation of four pear cultivars. in the first trial, the effect of four cultural media (ms, ms/2, msn/2 and mql) were compared on such traits as shoot length, shoot number and color of leaf. from among the media, ms/2 and msn/2 resulted in the most shoot proliferation and the longest shoots respectively. ms medium produced green developed leaves as compared to msn/2, and mql medium resulted in severe chlorosis accompanied by decrease in shoot growth. for a more precise evaluation of media (the second trial), 10 namely including the previous ones plus their modified versions were tested while the leaf size being measured too. based upon the results of the first and second trials, ms/2 was the best medium among all. a third trial was conducted to determine the effect of ba concentration and auxin types on shoot proliferation of the evaluated cultivars in ms/2 medium. ‘shahmiveh’ and ‘dargazi’ produced the highest shoot numbers in 2 mg/l of ba concentration while, ‘williams’ and ‘natanzi’ produced the same in 3 mg/l of ba concentration. equal concentrations of iba and naa (0.1 mg/l) in combination with ba showed that iba led to the production of longer shoots than naa.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.)

Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant reg...

متن کامل

Protein Synthesis and Breakdown during Heat Shock of Cultured Pear (Pyrus communis L.) Cells.

Cultured pear (Pyrus communis L. cv Passe Crassane) cells were subjected to temperatures of 39, 42, and 45[deg]C. Heat-shock protein (hsp) synthesis was greater at 30[deg]C than at temperatures above 40[deg]C and continued for up to 8 h. Both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. Polysome levels decrease...

متن کامل

The Draft Genome Sequence of European Pear (Pyrus communis L. ‘Bartlett’)

We present a draft assembly of the genome of European pear (Pyrus communis) 'Bartlett'. Our assembly was developed employing second generation sequencing technology (Roche 454), from single-end, 2 kb, and 7 kb insert paired-end reads using Newbler (version 2.7). It contains 142,083 scaffolds greater than 499 bases (maximum scaffold length of 1.2 Mb) and covers a total of 577.3 Mb, representing ...

متن کامل

Modulation of antioxidant compounds in organic vs conventional fruit (peach, Prunus persica L., and pear, Pyrus communis L.).

Despite the increasing interest in organic products, knowledge about how different levels of fertilization affect nutritionally relevant components is still limited. The concentration of polyphenols and the activity of polyphenoloxidase (PPO), together with the content in ascorbic acid, citric acid, and alpha- and gamma-tocopherol, were assayed in conventional and organic peach (Prunus persica ...

متن کامل

The Relationship between the Morphology and Structure and the Quality of Fruits of Two Pear Cultivars (Pyrus communis L.) during Their Development and Maturation

The flavour and nutritional values of pears are appreciated by consumers worldwide, who, however, demand specific fruit quality, that is, attractive appearance, firmness and flavour, and health safety as well as long-term shelf life and storability. Pear cultivars differ in terms of the above-mentioned traits; therefore, we undertook investigations to demonstrate the differences in structure of...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
علوم باغبانی ایران

جلد ۴۰، شماره ۲، صفحات ۰-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023